Official websites use. Share sensitive information only on official, secure websites. Breast cancer is a heterogeneous disease. Tumor cells and associated healthy cells form ecosystems that determine disease progression and response to therapy. To characterize features of breast cancer ecosystems and their associations with clinical data, we analyzed human breast tumor and 50 non-tumor tissue samples using mass cytometry. The expression of 73 proteins in 26 million cells was evaluated using tumor and immune cell-centric antibody panels.
Tumors displayed individuality in tumor cell composition, including phenotypic abnormalities and phenotype dominance. Relationship analyses between tumor and immune cells revealed characteristics of ecosystems related to immunosuppression and poor prognosis.
This large-scale, single-cell atlas deepens our understanding of breast tumor ecosystems and suggests that ecosystem-based patient classification will facilitate identification of individuals for precision medicine approaches targeting the tumor and its immunoenvironment.
Keywords: breast cancer, tumor ecosystem, tumor heterogeneity, immunosuppression, T cell, macrophage, single-cell analysis, mass cytometry.
Single-cell proteomics reveals tumor and immune cell diversity in tumor ecosystems. Breast cancer exhibits tumor cell phenotypic abnormalities and tumor individuality. Tumor-immune relationships in the tumor ecosystem are patient-stratifying. A single-cell atlas of cancer and immune cells reveals distinct tumor ecosystems across breast cancer patients, informing prognosis and, potentially, therapy selection.
